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Skeletal Box plot representation of <t>cell</t> viability. The sVW-MSCs were cultured in PGM without <t>growth</t> <t>factor</t> supplementation (PGM), with 10% FBS (PGM +FBS), with MSC <t>specific</t> growth factors (PGMadd), with equine PL (PGM PL H), or with swine PL (PGM PLs S). The median is plotted in black as a line, the 1 st and 3 rd quartiles as a dashed box, and the maximum and minimum values as whiskers with end caps. The mean values ± SE (standard error) are reported in blue. FBS: fetal bovine serum; PGM: perycite growth medium; PLs H: platelet lysate serum of the horse: PLs S: platelet lysate serum of swine. Pair comparisons with a significance level using the Steel-Dwass-Critchlow-Fligner method and median difference estimations are indicated on the right.
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Skeletal Box plot representation of cell viability. The sVW-MSCs were cultured in PGM without growth factor supplementation (PGM), with 10% FBS (PGM +FBS), with MSC specific growth factors (PGMadd), with equine PL (PGM PL H), or with swine PL (PGM PLs S). The median is plotted in black as a line, the 1 st and 3 rd quartiles as a dashed box, and the maximum and minimum values as whiskers with end caps. The mean values ± SE (standard error) are reported in blue. FBS: fetal bovine serum; PGM: perycite growth medium; PLs H: platelet lysate serum of the horse: PLs S: platelet lysate serum of swine. Pair comparisons with a significance level using the Steel-Dwass-Critchlow-Fligner method and median difference estimations are indicated on the right.

Journal: International Journal of Veterinary Science and Medicine

Article Title: Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate

doi: 10.1080/23144599.2024.2380586

Figure Lengend Snippet: Skeletal Box plot representation of cell viability. The sVW-MSCs were cultured in PGM without growth factor supplementation (PGM), with 10% FBS (PGM +FBS), with MSC specific growth factors (PGMadd), with equine PL (PGM PL H), or with swine PL (PGM PLs S). The median is plotted in black as a line, the 1 st and 3 rd quartiles as a dashed box, and the maximum and minimum values as whiskers with end caps. The mean values ± SE (standard error) are reported in blue. FBS: fetal bovine serum; PGM: perycite growth medium; PLs H: platelet lysate serum of the horse: PLs S: platelet lysate serum of swine. Pair comparisons with a significance level using the Steel-Dwass-Critchlow-Fligner method and median difference estimations are indicated on the right.

Article Snippet: The VW-MSCs were thawed, seeded and routinely recovered in primary culture flasks (1.5 × 10 6 cells/T75-flask) in PGM (Promocell, Heidelberg, Germany) to which 1× antibiotic-antimycotic solution and vascular stem cell specific growth factor mix (Promocell) was added to constitute the complete PGM medium.

Techniques: Cell Culture